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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.

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This information outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...

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This information outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is particular.

Focus on Info Cyclin E belongs into the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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